Cloning and Expression of VP2 Outer Coat Protein Gene of Bluetongue Serotype1 Virus, Indian Isolate
نویسندگان
چکیده
Bluetongue (BT) is a viral disease causing morbidity and mortality in sheep, cattle and wild ruminants, including deer, sambar and bluebull. In this study we present the internal gene of outer coat protein, VP2 of bluetongue virus serotype 1 of Indian isolate was amplified from cDNA synthesized through RT-PCR using total RNA with specific forward and reverse primers. The PCR product was cloned to pBKS cloning vector and sequenced. It was found that 880bp VP2 gene was 87% homology to Australian BTV VP2 and 85% homology to BTV serotype 1. The VP2 gene was released from pBKS cloning vector and sub cloned into expression vector pET32a and expressed protein of size 32.89 KDa was confirmed by SDS-PAGE and Western blot analysis further the expressed antigen could be used for diagnosis of BT without cross reaction and for vaccination once the antigen evaluated through clinical trials.
منابع مشابه
Cloning and Expression of the Coat Protein Gene of Barley Yellow Dwarf Virus-PAV in Escherichia coli
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